The increase in CD8+IFN-γ+ T cell infiltration (Fig. 2h) was further reflected in the dramatic increase in the ratio of CD8+IFN-γ+ T cells to Treg cells (Fig. 2i) at the tumor site by anti-4-1BB in comparison with combination therapy, which correlated with improved tumor inhibition by combination therapy (Fig. 2a, b). This evidence concerns the gene CD8A and neoplasm.