The spermatid heads were labeled with ProtamineA-GFP and cyst cell membrane was labeled with PpY>mCD8-RFP in both the control (ProtA-GFP/UAS-Dicer; PpY-Gal4>UAS-mCD8-RFP/+) and dlg1 RNAi (ProtA-GFP/UAS-dsDlg1; PpY-Gal4>UAS-mCD8-RFP/+) backgrounds. This evidence concerns the gene DLG1 and cyst.