LEP and polycystic ovary syndrome: Past analyses demonstrated that patients diagnosed with PCOS did not show any mutation of the leptin coding exons, while the amino acid variants in exon 2, 4 and 12, through a single-stranded conformational polymorphism (SSCP) analysis, followed by sequencing, did not show any association between leptin and PCOS [40], suggesting that much more insightful evidence should be gained by further studies.