ACTA1 and dry eye syndrome: NLRP3 with F4/80 in KCs or with α-SMA in HSCs were labelled by immunofluorescence double staining (Fig. 6c), and NLRP3, Caspase 1 and IL-1β were all detected in KCs and HSCs by RT-PCR and western blotting (Fig. 6d, e).