In the present work, in order to provide meaningful information about the subcellular localization of peIF2α and eIF2α, we performed studies with specific antibodies against the phosphorylated (peIF2α) and the total eIF2α form (eIF2α) comparing metastatic melanoma hmel1 and M3 (V600BRAF) with HBL cell lines (wtBRAF). The gene discussed is EIF2A; the disease is metastatic melanoma.