Our data showed that PD-L1 had significantly higher expression levels in the higher-TMB subtype than in the lower-TMB subtype of 7 cancer types (STAD, COAD, BLCA, SKCM, PAAD, LUAD, and CESC), compared to 3 cancer types (KIRP, GBM, and UVM) of which PD-L1 was more lowly expressed in the higher-TMB subtype (Student’s t test, P < 0.05, fold change > 1.5) (Fig. 6b). This evidence concerns the gene CD274 and cancer.