We also performed a multivariate analysis including the known prognostic parameters age (younger or older than 60 years), Flt3-ITD, NPM1 insertions, favorable and adverse/intermediate cytogenetics and disease etiology (secondary versus de novo AML) in addition to the number of colonies (below or above 200 colonies) (Table 3). Here, NPM1 is linked to acute myeloid leukemia.