Results from qMSP indicated that the CpG-rich regions of LDOC1 promoter, mLDOC1-2 and mLDOC1-4, were apparently methylated in lung cancer cell lines A549, H1355, and H1299, with additional methylated mLDOC1-1 presented in H1299, which show LDOC1 as completely silenced. The gene discussed is LDOC1; the disease is lung cancer.