H3K27 acetylation detected at analyzed regions of the PTBP3 locus in prostate cancer cells (PC3 and PC3M) may suggest that open chromatin regions are located at positions −63 kb and +48 kb of the PTBP3 promoter, respectively, and may act as epigenetic activation regulators of the PTBP3 gene. This evidence concerns the gene PTBP3 and prostate carcinoma.