FLOT1 and Familial prostate cancer: As shown in Supplementary Fig. S4a, IGF-1 induced PC3 cell proliferation, and the proliferation was increased by WT Flot-1 or KR mutant, but not CA mutant overexpression, suggesting that palmitoylation, but not sumoylation, of Flot-1 is responsible for IGF-1R signaling-mediated prostate cancer cell proliferation.