Histological analysis revealed that TS1 + WT-CAF or TS1 + KO-REC-CAF tumours shared a poorly differentiated morphology and invasive phenotypes typical of high-grade tumours, with loss of basal lamina (i.e. laminin staining), despite having similar number of αSMA-positive CAFs (Fig. 5d). This evidence concerns the gene LAMB2 and neoplasm.