AMER1 and colorectal carcinoma: To further investigate the clinical relevance of the miR-20a/106a/WTX/RhoGDIα/CDC42 signaling pathway in CRC progression, the 3D invasion experiments had performed and shown that inhibition of miR-20a/miR-106a could inhibit CRC cell 3D sphere growth and invasion, while overexpression of miR-20a/miR-106a could improve CRC cell 3D sphere growth and invasion (Supplementary Figure 7a, b, p < 0.001).