To determine if TNFR1 and TNFR2 expression is different on nTreg from on iTreg when the mice suffer from autoimmune diseases, we also analyzed their expression on nTreg of WT, TNFR1−/−, and TNFR2−/− mice and compared the two TNFRs on naive and disease mice. The gene discussed is TNFRSF1B; the disease is autoimmune disease.