Our study has confirmed the influence of TNF/TNFR2 on Tregs, and made a further step on how the two TNFRs impact on Treg proliferation and function, especially Tregs induced in vitro, which may overcome the paucity of nTreg and provide a new tool to optimize Treg cell therapy for autoimmune diseases. This evidence concerns the gene TNFRSF1B and autoimmune disease.