CYP24A1 is a key enzyme that is involved in the catabolism of vitamine D. It can catalyze 25-OH-D3 and 1α,25-(OH)2D3 into 24-hydroxylated metabolites26.To evaluate the correlation between the expression of CYP24A1 and the malignancy of glioma, and the expression pattern of CYP24A1 in acidic microenvironment in vivo is also important. This evidence concerns the gene CYP24A1 and glioma.