To investigate whether Fn infection and its downstream target genes can affect the efficacy of 5-Fu-based chemotherapy in CRC patients, we initially detected the Fn abundance and BIRC3 and TLR4 expression levels in FFPE CRC tissues from patients who were pathologically diagnosed with advanced CRC and received standard 5-Fu-based adjuvant chemotherapy after radical surgery. The gene discussed is FN1; the disease is colorectal carcinoma.