Furthermore, we dissected the mechanisms involved in Fn-induced BIRC3 expression, which showed that the TLR4/NF-κB pathway regulates BIRC3 expression in CRC cells that are cocultured with Fn. Finally, to correspond to the aforementioned findings, we investigated the clinical significance of Fn infection and its downstream target genes. Here, NFKB1 is linked to colorectal carcinoma.