Since monomeric Ln-γ2, rather than heterotrimeric Ln-γ2, is expressed preferentially in HCC nodules, we have evaluated the diagnostic value of monomeric Ln-γ2, AFP, and PIVKA-II in sera from patients with HCC and chronic liver diseases (CLD) using the above-mentioned automated CLIA along with ARCHITECT [22]. The gene discussed is AFP; the disease is hepatocellular carcinoma.