Collectively, the ECIS and transwell experiments indicate that TLR3-deficient OE cells are more susceptible to Chlamydia infection-induced breakdown of the monolayer integrity, and suggest that TLR3 signaling plays a substantial role in the maintenance of epithelial barrier function in oviduct epithelium during Chlamydia infection. Here, TLR3 is linked to chlamydia infectious disease.