To ascertain whether TLR3’s function indeed has a role in the chlamydial spread and ascension into the upper genital tract (UGT) tissue of female mice, we infected WT and TLR3-deficient (TLR3-KO) mice with 105 IFU C. muridarum and harvested the UGT tissue from mice at either day 3, 5, or 7 post-infection. Here, TLR3 is linked to infection.