Because ABCB8 downregulation induces iron-mediated ROS production in cardiomyocytes and melanoma cells (Ichikawa et al., 2012, Ichikawa et al., 2014), we investigated whether ABCB8 regulates iron-dependent oxidative stress by measuring mitochondrial superoxide level in HDMECs expressing or lacking ABCB8 and treated with deferoxamine. The gene discussed is ABCB8; the disease is melanoma.