Considering that the binding of tropomyosin and myosin to actin is a key mechanism for regulating the normal function of the complex (Rayment et al., 1993; Behrmann et al., 2012) and that an alteration in actin filament regulation is an important factor in deafness caused by ACTG1 mutations, these de novo variants likely alter the protein activity, leading to deleterious effects (Lee et al., 2018). This evidence concerns the gene ACTG1 and deafness.