Reagents that regulate T cell metabolism (e.g., 2-DG, ETX, etc.), inhibit IL-2 activity or augment PD-L1/PD1 interactions in GVHD target tissues could tolerize infiltrating T cells while maintaining the function of T cells in lymphoid tissues, such that GVHD could be effectively prevented while strong GVL activity is preserved. The gene discussed is CD274; the disease is graft versus host disease.