Compared with previous study at a single time point, the present study further revealed that the mRNA level of IRAK-M was dramatically upregulated at 1 h after cerebral ischemia reperfusion, and gradually downregulated in 24 h in the ischemic hemisphere, suggesting IRAK-M plays a role in acute stage of ischemic stroke. The gene discussed is IRAK3; the disease is brain ischemia.