TGFB1 and neoplasm: We then tested the in vitro induced T-cell responses by co-culturing GM15-2 step DCs or DC5 (prepared from bone marrow cells of tumor bearing animals) with T cells derived from ascites, spleen or draining lymph nodes of tumor bearing animals for 24 h and subsequently measuring levels of IFNγ, IL-4, TGFβ and IL-10 in the culture supernatant by ELISA.