In order to compare their immunogenic phenotype, we first differentiated DCs in vitro from the bone marrow of tumor bearing animals with GM-CSF and IL-4 (GM4-1 step DCs) or with GM-CSF and IL-15 (GM15-1 step DCs), pulsed with a squaric acid treated-ID8 tumor cell lysate (LSQ) and finally matured them with LPS plus IFNγ. The gene discussed is CSF2; the disease is neoplasm.