Our results demonstrate that DCs differentiated with GM-CSF and IL-15 and matured in the presence of anti-CD40, anti-PGE2, anti-PGE2 receptor (EP2), anti-IL-10 receptor antibodies and CpG oligonucleotides (CpG) (in addition to the canonical LPS and IFNγ) exhibited higher levels of anti-tumor response compared to canonical DC formulations. This evidence concerns the gene CSF2 and neoplasm.