We then examined whether the isoflavonoids induce, i) ERE-dependent luciferase gene transcription in MCF-7:D5L cells (known to express ERα), ii) expression of AlkP in Ishikawa endometrial adenocarcinoma cells (known to express ERα and ERβ), iii) stimulate proliferation of MCF-7 breast adenocarcinoma cells (known to express ERα but not ERβ) and, iv) affect proliferation of MDA-MB-231 breast adenocarcinoma cells (known to express neither ERα nor ERβ). This evidence concerns the gene ESR1 and breast adenocarcinoma.