Although the hyperglycaemia and hyperphosphatemia present in many CVD patients is known to suppress SIRT1 expression, there is a growing need for a comprehensive single-cell differentiation pathway for vSMC phenotypic switching, to identify the temporal activity of relevant signaling pathways, and the importance of their early inhibition and reactivation at later time-points. Here, SIRT1 is linked to Hyperglycemia.