In summary, as shown in Graphical Abstract, our findings provide evidence that MALT1 protease activity, known to play an important role in NF-κB activation and pathogenesis of ABC-DLBCL, promotes glutaminolysis by up-regulating GLS1 expression and facilitates the generation of PD-L1+ ABC-DLBCL cells. This evidence concerns the gene MALT1 and diffuse large B-cell lymphoma.