TACSTD2 and gelatinous drop-like corneal dystrophy: Here, we establish a new in vitro disease model of GDLD based on immortalized human corneal epithelial (HCE-T) cells.<h4>Methods</h4>By using transcription activator-like effector nuclease plasmids, tumor-associated calcium signal transducer 2 (<i>TACSTD2</i>) and its paralogous gene, epithelial cell adhesion molecule (<i>EpCAM</i>), were knocked out in HCE-T cells.