To extend our findings to an infectious challenge we labeled tachyzoites of the parasite N. caninum with carboxyfluorescein succinimidyl ester [(CFSE); Nc-CFSE] and challenged CD14+ monocytes with Nc-CFSE at a multiplicity of infection (MOI) of 4 for 24 h and subsequently infected cells were identified using FACs analysis. This evidence concerns the gene CD14 and infection.