Indeed, feeding of mice with HFD resulted, within 12 weeks, in the development of a diabetic phenotype (significant weight gain, impaired glucose tolerance, increased fasting blood glucose and increased fasting plasma insulin) and diabetic cardiomyopathy (reduction in %EF) as a result of NF-κB activation in the heart (see below). Here, NFKB1 is linked to Impaired glucose tolerance.