ANXA5 and plasma cell myeloma: To this end, P3X63Ag8.653 myeloma cells obtained from standard culture (and thus without stress signals) were double-stained with DAPI and Annexin V and arbitrarily subdivided into populations designated as “live” (Annexin V− DAPI−), “apoptotic” (Annexin V+ DAPIlow), “necrotic” (Annexin V+ DAPI+), and “dead” (Annexin V− DAPI+) (Figure 4A).