Combined results of western blot and immunohistochemistry showed that cos could inhibit LPS/D-GalN-induced ALI via NF-κB signaling pathway by up-regulating the levels of TLR4, MyD88, p65 (Nucleus), p-IKKα/β, IKKα/β, and p-IκBα proteins and down-regulating the levels of p65 (Cytosol) and IκBα protein. The gene discussed is MYD88; the disease is acute respiratory distress syndrome.