Because it is known that MMPs play a major role in promoting metastasis of cancer cells (Deryugina and Quigley, 2006; Halbersztadt et al., 2006; Alaseem et al., 2017), we first examined the proteolytic activity and expression level of MMP-2 and MMP-9 in ISLA-treated and untreated HT1080 CM. Here, MMP9 is linked to cancer.