Similar results were observed in the rectal cancer cell line SW837, where the depletion of DOT1L (Additional file 1: Figure S1a, b) also led to decreased induction of γH2AX 15 min after DSB induction (Fig. 1d), while pKAP1 levels were elevated compared to control cells (Fig. 1d, Additional file 1: Figure S1d). Here, DOT1L is linked to rectal cancer.