NLRP3 and Down syndrome: Therefore, to confirm the role of IL-1β in the kidney and liver and to analyze, for the first time, the potential protective effect of Canakinumab, a monoclonal antibody targeting IL-1β currently used for the treatment of immune-inflammatory diseases (such as rheumatoid arthritis, systemic juvenile idiopathic arthritis, and syndromes associated with cryopyrin), we treated epithelial cells of the proximal renal tubule (HK-2) and hepatic stellate cells (LX-2) with this cytokine and subsequently measured EMT markers using various classical molecular biology techniques.