To further verify the expression, we detected the PPP1R3C coded protein expression with western blot, results showed that PTG significantly increased in NAFLD group in comparison to Normal group, while the protein expression markedly decreased by LGZG treatment (Fig. 2g), indicating PPP1R3C was a potential target of LGZG decoction in improving NAFLD. The gene discussed is PPP1R3C; the disease is metabolic dysfunction-associated steatotic liver disease.