To verify the role of LGZG on hepatic de novo lipogenesis, we detected SREBP-1c, the master molecule that responsible for de novo lipogenesis, and found that SREBP-1c protein expression in nucleus significantly increased in NAFLD rats compared to Normal rats, and LGZG intervention significantly decreased the nucleic level of SREBP-1c (Fig. 5a). The gene discussed is SREBF1; the disease is metabolic dysfunction-associated steatotic liver disease.