To determine if F10 [Figure 1] displays improved cytotoxicity relative to 5-FU in cellular models of CRC and to evaluate the effects of p53 mutation on drug response, F10 and 5-FU were tested in the human CRC cell line, HCT-116 and three isogenic cell lines derived from HCT-116 that differ in p53 status. Here, TP53 is linked to colorectal carcinoma.