In the present study, we found downregulation of Wnt canonical target Cyclin D1 and Survivin in both 143B and SJSA-1 cells after treatment with PRI-724 (Figure 2C, 4B; Supplementary Figure 1), suggesting that PRI-724 acts in a Wnt-dependent manner in the context of osteosarcoma. This evidence concerns the gene CCND1 and osteosarcoma.