For flow cytometry analysis, the human grade IV glioma cells were treated for 24 h with different concentrations of MtRV extract (0.25, 0.5, 1, 1.5 mg mL−1) or DMSO (0.1–0.3%), for controls, and then stained with Annexin-V-FITC and PI. The gene discussed is ANXA5; the disease is central nervous system cancer.