Our findings demonstrate that polyphenolic compounds (e.g. ellagic acid, hydroxybenzoic acid, syringic acid, sinapinic acid, chlorogenic acid, ferulic acid or rutin) and terpenoids (betulinic acid) present in this extract can exert a synergistic effect and induce apoptosis by G2/M phase cell cycle arrest, change the protein expression level of Bax, Bcl-2, Cas-3 and p53 and decreased mitochondrial membrane potential in grade IV glioma cells. The gene discussed is EFS; the disease is glioma.