TWIST1 and cancer: To recognize the cancer cells with EMT phenotype, we have performed immunostaining for CDH1, CDH2, NAT1, SNAI2, TWIST1, VIM, and ZEB1. We were not able demonstrate the significant difference between EMT-activated and not-EMT-activated group regarding CDH1, CDH2, SNAI2, VIM, and ZEB1 expression, in the tumor bulk as well as at the invasive edges of the tumor tissue.