CD34 and chronic myelogenous leukemia, BCR-ABL1 positive: To analyze the role of aPKCι in human LSC/P survival and maintenance in vivo, CML CD34+ cells were transduced with control or aPKCι shRNA lentiviral vector followed by transplantation into sub-lethally irradiated NSG-SGM3 humanized mice (Fig. 6a).