When the ESCC cells were treated with 12-O-tetradecanoylphorbol 13-acetate (TPA), a regular substitution of diacylglycerol (DAG), results showed it could rescue the side effect of PLCE1 knockdown in NF-κB-related proteins, phosphorylated-IKK-α/β, −p65, and-IκBα protein. Here, NFKBIA is linked to esophageal squamous cell carcinoma.