In order to choose the appropriate concentration of TGF-β1 to induce EMT and ViceninII to avoid cell death in subsequent experiments, the cytotoxicity of ViceninII (1.25, 2.5, 5, 10, 20, 40, and 80 μM) and TGF-β1 (0.625, 1.25, 2.5, 5, 10, 20, and 40 ng/mL) on lung adenocarcinoma A549 and H1299 cells was measured by MTT [3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazoliumbromide] assay after 24 h and 48 h. Here, TGFB1 is linked to lung adenocarcinoma.