Therefore, these new established cells could be considered as a source for autologous therapy in HIV infection.20 To overcome little activity of CRISPR system in CD4+ T cells, it is possible to utilize a dual gRNA approach for inducing biallelic deletion in CCR5 gene and consequently, improve the disruption of CD4+ T cells and CD34+ HSPCs.21 Recently, Cas9 ribonucleoprotein (RNP) complex has been used to target host factors that are involved in HIV infection. This evidence concerns the gene RNPC3 and HIV infectious disease.