Further, The Cancer Genome Atlas (TCGA) data set of 844 patients with invasive breast carcinoma (RNA sequencing version 2 analysis [RNA Seq V2] normalized gene expression with RSEM output [RSEM]) revealed a correlation between PDPN mRNA level and LN metastasis, showing higher levels of PDPN mRNA (PDPN mRNA expression normalized to Gapdh) associated with more LN metastasis (Fig 5A). This evidence concerns the gene PDPN and invasive breast carcinoma.