Using the human bladder cancer cell lines 5637 and T24, we isolated BCSCs by culturing 5637 or T24 cells in serum‐free DMEM/F12 (1:1) containing B27, recombinant EGF at 20 ng/mL and recombinant bFGF at 10 ng/mL. Here, EGF is linked to urinary bladder carcinoma.