As shown in Figure 2F, the luciferase signal in si‐LncRNA group was typically lower than the si‐NC group (P < 0.05), similarly, the tumor weight in si‐LncRNA group was obviously lower than the si‐NC group (P < 0.05, Figure 2G).Taken together, we concluded that down‐regulated LncRNA NR2F2‐AS1 contributed to the promotion of cell apoptosis and the inhibition of cell proliferation and invasion in A549 and SPC‐A‐1 cells in vivo and vitro. Here, NR2F2 is linked to neoplasm.