We found that there was a significant enrichment of pgc mRNA bound to Me31B-GFP protein comparable to those of the positive control, osk mRNA (Figure 4A) (Nakamura et al., 2001) and no significant enrichment of a non-target RNA, ileRS. Next, we assayed for pgcGFP expression upon germline depletion of me31B and found a loss of GFP repression from the GSC to the 4-cell cyst (Figures 4B–4C1 and 4E). This evidence concerns the gene PGC and cyst.