To explore the possible role of the estrogen receptor in E2-mediated amelioration of anxiety and depressive phenotypes in our mouse model, we examined its expression in the hippocampus, but we found that the levels of mRNA for estrogen receptor α, estrogen receptor β, and a G protein-coupled receptor (GPR30) were indistinguishable among the 4 groups of mice (S2 Fig). Here, ESR1 is linked to Anxiety.