GSDMD and infection: Thus, as we observed that B. abortus is able to trigger pyroptosis in macrophages, we assessed the requirement of GSDMD for pore formation in response to B. abortus. First, BMDMs obtained from C57BL/6, Casp11-/-, Gsdmd-/- and Casp1/11-/- mice were left uninfected or infected with B. abortus. By evaluating propidium iodide uptake in real time during 8 h of infection, we found that BMDMs from C57BL/6 mice formed pores whereas BMDMs from Gsdmd-/-, Casp11-/- and Casp1/11-/- mice failed to form pores in response to B. abortus (Fig 7B).