Based on the high expression of EphrinB2 in liver cancer cell lines, we used MTT and colony formation assays to examine the effect of B10 on cell proliferation and colony formation in a panel of liver cancer cell lines, including Hep3B, SMMC‐7721, HepG2, Bel‐7402 and 97 h cells. This evidence concerns the gene EFNB2 and liver cancer.