HMGB1 and myocardial infarction: Other endogenous ligands such as high mobility group box 1 or heat‐shock proteins which are released or secreted following trauma have been shown to modulate myocardial function.30, 31, 32 Thus, tissue damages caused by tracheal intubation and chest‐opening not only threaten animal life, but also affect the accuracy of observations on the traditional MI model.33, 34 Gao et al14, 15 have developed a rapid surgical method to ligate LCA without ventilation, which improved the short‐term and long‐term survival rates in mice.